![]() Data were analyzed using FlowJo software (TreeStar. ![]() Taken together, our data suggest that combining WFA with Dox decreases the dosage requirement of Dox, therefore, minimizing/eliminating the severe side effects associated with high doses of DOX, suggesting the application of this combination strategy for the treatment of ovarian and other cancers with no or minimum side effects. After washing three time, cells were fixed and analyzed using a FACSCalibur flow cytometer (BD Biosciences). Immunohistochemical analysis of the tumor tissues from animals treated with WFA/Dox combination showed a significant reduction in cell proliferation and formation of microvessels accompanied by increased in LC3B level, cleaved caspase 3, and DNA damage. Both tumor models showed a 70 to 80% reduction in tumor growth compared to control or animals treated with WFA or Dox alone. We validated combination therapy on tumor growth using an in vitro 3Dimension (3D) tumor model and the more classic in vivo xenograft model of ovarian cancer. Combination treatment resulted in a significant enhancement of ROS production resulting in immense DNA damage, induction of autophagy analyzed by transmission electron microscope and increase in expression of autophagy marker LC3B, and culminated in cell death analyzed by cleaved caspase 3. synergistic effect on inhibition of cell proliferation and induction of cell death, thus reducing the dosage requirement of Dox. ![]() Treatment of various epithelial ovarian cancer cell lines (A2780, A2780/CP70 and CaOV3) with combination of WFA and Dox (WFA/DOX) showed a time- and dose-dependent. Sequencing was performed using 1 SMRTcell 8M per pair of barcoded samples on a Sequel IIe system in the UofL Sequencing Technology Center. We used combination strategy by combining doxorubicin (Dox) with withaferin A (WFA) to minimize the ill effects of Dox. The University of Louisville is a public university in Louisville, Kentucky, a member of the Kentucky state university system.Application of doxorubicin (Dox) for the treatment of cancer is restricted due to its severe side effects. Learn more Address: 2301 S 3rd St, Louisville, KY 40292, USA Phone: +1 50 Undergraduate enrollment: 14,844 (2015–16).See all rates Average annual cost is for first-time, full-time undergraduate students receiving federal aid Source: US Dept of Education.They were the largest group of students (71%) according to the 2015–2016 College Scorecard data See all rates Graduation rate is for first-time, full-time undergraduate students who graduated within six years.When founded in 1798, it was the first city-owned public university in the United States and one of the first universities chartered west of the Allegheny Mountains.Īvg cost after aid $16K Graduation rate 54% Acceptance rate 72% more Graduation rate is for first-time, full-time undergraduate students who graduated within six years. University of Louisville List of Employees There's an exhaustive list of past and present employees! Get comprehensive information on the number of employees at University of Louisville. You can filter them based on skills, years of employment, job, education, department, and prior employment. You can even request information on how much does University of Louisville pay if you want to. Learn about salaries, pros and cons of working for University of Louisville directly from the past employees.įind People by Employers You can rekindle an old relationship, reconnect with a long-lost friend, former boss, business acquaintance who might be useful in your new line of work. With our employee database, the possibilities are endless. All you have to do is type in a couple of keywords and we'll bring you the exact information you wanted!ĥ,483 University of Louisville employees in database. ![]() Find out everything there's to know about University of Louisville employees. ![]()
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